Ileal Villi Morphological Characteristics of Cobb 500 Broilers Fed Phytase and Tannase Treated Sorghum Based Diets

The effects of phytase and tannase enzyme treatment sorghum-based broiler diets on ileal villi characteristics were evaluated in Cobb 500 broilers. A total three sorghum levels, 0, 50, 100%, with 4 levels; 5% phytase, phytase+tannase combination used to develop 12 different dietary treatments. Three hundred sixty broilers randomly allocated the treatments a completely randomized design experiment. On day 42, two birds from each replicate selected slaughtered for morphometry analyses. 2cm tissue sample ileum was cut prepared histological Villus height width, muscularis externa thickness, crypt depth measured light microscope using calibrated eyepiece graticule. surface area calculated, which indicates digestive absorptive capacity ileum. General Linear Models (GLM) procedure Statistical Analysis System ver 9.4 (SAS Institute Inc., 2011) analyse data. All tests performed at p < 0.05 significance. height, thickness significantly increased increasing levels diet (p